Singh, Sasha A.

Assistant Professor of Medicine, Harvard Medical School;
Associate Scientist, Brigham and Women's Hospital;

Director of Proteomics Research, CICS

(617) 730-7702

sasingh@bwh.harvard.edu

Dr. Sasha Singh earned her MSc in Developmental Genetics (2001) and PhD in Biochemistry/Protein Crystallography (2007) from the Department of Cell and Systems Biology at the University of Toronto (2007). Following her doctoral studies, she joined the labs of Steen and Steen at Children’s Hospital Boston and Harvard Medical School for her postdoctoral studies in mass spectrometry guided research. During this time, Dr. Singh contributed to several projects, including high-throughput proteomics screens and detailed investigations of protein post-translational modifications in relation to the cell cycle, cancer, and several neurological disorders. A highlight of her work during this time was the development of FLEXIQuant, a practical and effective workflow for implementing full-length protein mass spectrometry standards, which later evolved into a novel in vitro kinase assay known as FLEXIQinase.

Dr. Singh joined the Center for Interdisciplinary Cardiovascular Sciences in 2012 and has been an innovative leader ever since. She brings a diverse skill set, seamlessly integrating qualitative and quantitative mass spectrometry-guided experiments into the center’s research efforts. Her work has contributed significantly to the development of foundational research and target discovery platforms focused on understanding and tackling mechanisms of cardiovascular disease. Notably, Dr. Singh was the first to apply high-resolution, high-accuracy mass spectrometry to monitor protein turnover in humans. Her work has contributed to a paradigm shift in our understanding of “good HDL-cholesterol particle” metabolism, providing insight on why HDL-cholesterol-raising trials have failed to reduce major adverse cardiovascular events. Dr. Singh also actively investigates the effects of various post-translational modifications on protein function. Her lab has extensive expertise in developing bench-to-instrument workflows for ADP-ribosylation proteomics, advancing the study of this critical modification and its role in cellular processes. 

Dr. Singh is a member of the American Society for Mass Spectrometry, the US Human Proteome Organization, the American Heart Association, and the International Society for Applied Cardiovascular Biology.

Publications